It is obvious that biosensing membranes with covalently bound enzymes [ 45, 46], additionally based on highly lipophilic [ 45] or insoluble [ 46] pH-indicative dyes, will exhibit significantly better operational stability.
The following factors contributed significantly to the total variance: G (gene; 5 %; Table 2), VG (validation experiment and gene interaction; 27 %), SG (slide and gene interaction indicative for dye-effects; 4 %; Table 2), and VSG (validation experiment, slides, and gene interactions; 31 %). a The degrees of freedom results in the separate ANOVAs are listed in the supplementary web-site [21].
HT29 proliferation was assessed by the Alamar Blue assay, which measures chemical reduction of the dye, indicative of metabolic activity of mitochondrial enzymes, which is determined from the fluorescent signal at 570 nm.
SmartAmp2 reaction mixtures were incubated at 60°C for 30 min under isothermal conditions using the 7500 Real-Time PCR Systems Applied Biosystemss, Forester City, CA, USA), where changes in the fluorescence intensity of SYBR Green I dye indicative of DNA amplification were monitored during the reaction.
Cell dyeing is indicative of cell membrane damage.
Due to the fact, that only vital cells are capable to metabolise XTT, the amount of produced formazan dye is indicative for the fraction of vital cells.
Enhanced quantity of dye adsorption is indicative of the enhanced surface area of TiO2 film for a monolayer of dye on the TiO2 particles.
On the other hand, Figure 10 shows that increasing concentration of the dye eventually generate high background signal, indicative of excessive presence of the dye.
This differentiation was qualitatively characterized by increased formation of cartilage nodules and enhanced staining with alcian blue dye, which is indicative of cartilage proteoglycan accumulation.
It was noted that although tetrazolium dye reduction is indicative of cellular respiration, it can occur independent of cell growth [ 29, 30].
The photochromic properties displayed by the nanoparticles are indicative of the successful incorporation of dyes into the nanoparticles.
