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Included studies showed a large heterogeneity of the indication score ranging between 1 and 4 points with a median (25%; 75% quartile) of 4 (2; 4).
As recommended by the RAM, we asked the panel members to rate the appropriateness of each indication (score them) based on their own professional judgment instead of what they perceived to be the views of other respected clinicians while considering the possible outcomes resulting from their decisions.
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No indication scored as appropriate by the panel was classified as inappropriate and vice versa.
Table 3 Table 4 LIPI-5: Information related to Drug Type, Action and Indication scored in different table The pre-publication history for this paper can be accessed here:.
The agreement rate was slightly higher for simple cataract than for the other two categories and the proportion of indications scored as appropriate also was slightly higher.
To minimise bias due to confounding by indication, propensity score matching therefore runs the risk of limiting the generalisability of the findings to younger and healthier patients.
Following the ESSG indications, a score ≥3.5 was considered to have a good sensitivity and specificity in separating patients with high DA level.
We also noted that ESR1 mRNA expression levels did not correlate strongly with either the ERS or luminal gene scores, an indication that these scores capture molecular information not provided by ESR1 expression levels alone (Additional file 4: Figure S7).
There were no indications of scoring errors attributable to stuttering or large allele dropout.
Quality control screening did not reveal indications of scoring error due to stuttering or evidence for large allele dropout.
Magnitude and direction of change were calculated for each item of the checklist for both indications (total scores for HSCL and GSCL) and for the global assessment of symptoms.
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