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The OD 260 280 ratio (measured by Nanodrop), frequently used as an indication of protein contamination, was within a range suitable for DNA analysis [ 9].
However, 1D gels may provide a superficial indication of protein recovery and cannot attest as to whether or not selective extraction of particular individual proteins may occur.
Western blot analyses of SKBr3 or MDA-MB-231 cells treated with NS1643 for up to 48 h revealed no detection of active caspase-3 or reduction of protein pro-caspase-3 density as indication of protein activation by cleavage.
After detecting via Western blotting, membranes were stained with an amido black solution (0.1% amido black [N3393; Sigma-Aldrich], 10% acetic acid, 25% isopropanol) to reveal all proteins as an indication of protein transfer.
Only the RNA samples with A260/A280 ratio (an indication of protein contamination) of 1.9 2.1 and A260/A230 ratio (an indication of reagent contamination) greater than 2.0 were used and 100 ng/ μL RNA was subjected to qRT-PCR and conventional RT-PCR assays.
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An indication of protein-degradation alteration (upregulation of Catepsin D, Hsp27, Ubiquitin conjugating enzymes) was also evident (Table 1).
The authors observed that SVGs encode proteins that are involved in adaptation to environmental stresses and proposed that highly variable domains are an indication of protein-protein interaction specificity or specificity of small molecule binding.
Nuclear protein localization studies give useful indications of protein function.
The protein measurement is intended only as an indication of changing protein concentrations and not intended as a strict protein quantification.
In order to gain indication of potential protein level changes of the proteins not identified in the proteomics study, relative quantification in real-time RT-PCR (qRT-PCR) was performed (Supplementary Table S2).
The most recurrent DNA inserts recovered from screen colonies are listed, with indication of the protein family they belong to, and publications linking the proteins to breast cancer development.
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