Sentence examples for indicating translation from inspiring English sources

The amount of active luciferase protein (indicating translation efficiency of the in vitro reaction) was measured using the Biotek 96-well-plate luminometer.

Traces of fluorescence as a function of time show substantial and sustained Cy3B fluorescence increase upon delivery of 50S subunits and SRP, indicating translation initiation as the 50S subunit bound stably to the PICs (green trace).

Yet a sizeable fraction (34%) of non-coding RNA smORFs displayed Poly-Ribo-Seq metrics above our cut-off values indicating translation and some can display FLAG and Western blot signal similar to annotated smORFs.

Only 164 annotated smORFs have at least two out of three markers indicating translation or peptide function: (1) molecular GO term indicating protein function (based on direct assays or presence of protein domains); (2) matches with peptides from proteomic experiments; and (3) conservation of the coding sequence beyond insects.

For medial-lateral (ML) translation, positive values indicate translation toward the MCL.

The CCDS guidelines stipulate that the longest ORF should be annotated unless there is compelling evidence that indicates translation initiation from an internal AUG is more likely.

Fourth, these authors performed a number of experiments that indicate translation initiation can begin upstream of the CGG repeat in the polyGly reading frame.

We have found 293 truncated copies of Satine and Kryze ORF1s that may be AVR/effector candidates (Table  3) and peptides that indicate translation of these genes (Table  4).

Polymerase chain reaction (PCR) amplification was performed using pGEM-T-NT3 plasmid DNa as a template with the following set of oligonucleotide primers: 5′XhoNtf3, 5′-ATC TTA CTC GAG AAC AAG GTG ATG TCC-3′, and 3′XhoNtf3, 5′-ATA T CT CGA GAC AGA TGC CAA TTC ATG-3′ (underlines indicate the XhoI site used for further construction, and bold indicates translation start site).

However, to the reviewer's point, we have added a line to the text stating that "Ribosome occupancy in this region may indicate translation of short regulatory polypeptides in upstream open reading frames (uORFs) or alternate production of N-terminal isoforms of canonically translated proteins... .......

Ribosome occupancy in this region may indicate translation of short regulatory polypeptides in upstream open reading frames (uORFs) or production of alternate N-terminal isoforms of canonically translated proteins (Ingolia et al., 2011; Lee et al., 2012; Stern-Ginossar et al., 2012).