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The operator verified the black terminal marker indicating tip integrity was present.
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High precisely captured optical images indicate tip radius wear effects on surface quality clearly.
AFM image of pattered PDMS substrate shown in Fig. 5B-a where pale yellow lines and reddish lines indicate tip positions and down parts, respectively.
A green arrowhead indicates tip cell.
Green and orange arrowheads indicate tip and stalk cells, respectively.
(A ) 3D-rendered time-sequential images of Tg(fli1 Gal4FF);(UAS:GCaMP7a);(fli1 H2B-mC) embryos at 31 ss when a EC fli1 H2B-mCip and stalk cembryos budding from the Dat Green and orange arrowheads indicate tip and stalk cells, respectively.
The spring constant of the tip is 48 N/m, indicating the tip has a high wear resistance, which aims to keep the sharpness of the tip unchanged during scratching.
Interestingly, tips with high cytoplasmic huDysGFP intensity near the tip do not necessarily recover more (compare Figure 6C,E,F) indicating real tip-to-tip variation in the dynamic bound pool.
These residues are not essential for SNX1-mediated membrane remodelling and similar positioned residues are also present in non-tubulating SNX5 (Supplementary Figure S7), indicating that tip-to-tip contacts of SNX1 are different from those observed in F-BARs.
"They are very, very short stories, like this," he says, indicating the tip of his index finger.
Temperature indicating paints (TIP) is a kind of special functional coating which measures the temperature distribution by judging the color changes of the paint film.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com