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Only the RiboPure™ kit resulted in a A260/230 ratio of 2.1 indicating samples free of organic compounds, followed by TRIzol® LS (1.3 ± 0.5), PAXgene™ (1.2 ± 0.6), RNeasy® (0.9 ± 0.6) and LeukoLOCK™ with the lowest ratio (0.8± 0.8±.
As expected, serum concentrations between the two visits one year apart were highly correlated for PBDE congeners with high detection rates, including BDE-28, 47, 99, 100 and 153, with Spearman correlation coefficients of 0.82-0.92 for the young children and 0.84-0.97 for the parents, indicating samples from a single time point can represent a longer time window of exposure.
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The Kaiser-Meyer-Olkin (KMO) score for the AES-C was 0.84, indicating sampling adequacy (≥.50).
Lanes 1 3 indicate control samples, lanes 4 6 indicate samples from mice subjected to OC administration.
Stars indicate samples from the representative IRM decomposition analyses.
Subscript s indicates samples and st indicates the standard material (sapphire).
Sample size estimates indicated samples of 14 subjects would detect '20% improvements in pain (α=.05, power=.80).
Triple asterisk denotes P < 0.001; indicated samples vs. uncoated control based on one way ANOVA test.
Asterisks indicate samples sequenced in this study; sequences of the other samples were cited from Chung et al. ([2014]).
Data are mean fold expression changes in indicated samples as compared to untransfected cells (Unt) ± SD (n = 3). Figure S2.
At times indicated, samples were taken from each culture, and the incorporation of radioactive material was determined.
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