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After spontaneous differentiation, loss of CLOCK protein due to Clock gene silencing induced spontaneous differentiation of mESCs, indicating an exit from the pluripotent state, or its differentiating ability.
The mRNA expression levels of the pluripotent markers Oct4, Sox2, Nanog, and Klf4 were gradually decreased in both the Clock knockout mESCs and wild type mESCs after spontaneous differentiation (Fig. 7A), indicating an exit from the pluripotent state.
A huge quantity of oxidized free fatty acid intermediates were found during turnover of 13-HOD by glyoxysomes from cucumber indicating an exit from β-oxidation during the first two rounds.
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"Looks like this one is open," he said, indicating an aisle seat on an exit row, one of the most desirable seats.
People liked it largely because it was bipartisan and indicated a responsible exit.
Pro tip: Yellow dots on the map mean a straightforward level, while red ones indicate a secret exit or two.
This indicates a delayed exit from cell cycle of progenitor cells and a delay of terminal differentiation, consistent with the ability of PC3/Tis21 to inhibit cell cycle and promote differentiation [4], [12], [16].
Three days treatment with 50 nM AZD8055 reduced Ki67 staining in both TamR and MCF7-X cells and after treatment with 100 nM 40% to 50% of all cells were deemed negative for MIB1 indicating a significant (P <0.05) exit from the cell cycle.
The internal consistency was high (Cronbach's alpha:.90) and there was a strong association between exit behavioural response and actual exit behavior, indicating a high degree of predictive validity.
But audiences gave Mr. Apatow's weakly reviewed movie a B-minus in exit polls, indicating a rough road ahead.
For both the SKN7 TFBS and the MCM1 TFBS, the PWM score is higher in BY relative to RM, perhaps indicating a larger connection between mitotic exit and osmotic stress in BY than in RM.
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