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The dotted line indicates proliferation rate of TEFF incubated without TREG.
A constant viability signal (in the range of the initial value) indicates inhibition of proliferation, whereas a signal increasing relative to the initial value (similar to untreated controls in most cases) indicates proliferation.
The presence of tetramer‐binding TAA‐specific CD8+ T cells after in vitro expansion, but not ex vivo (data not shown), indicates proliferation of a very low frequency precursor population.
The effect of Ephrin B3 siRNA on cell proliferation was examined using the carboxyfluorescein diacetate N-succinimidyl ester (CFSE) dye, which is diluted upon cell division and hence decreased CFSE signal indicates proliferation.
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While some reports indicate proliferation of Salmonella in the plant [7], [17], in other field studies, the population sizes of Salmonella steadily declined after inoculation of the plants.
(i) Adult neurogenesis has been assessed by different cell markers indicating proliferation (Ki-67), juvenile stages of neurons (DCX), and slowly dividing precursor cells (MCM2).
We observed that RF was twice fold higher in siRNA scr cells compared to siRNA Scd1-depleted U2OS 72 h post-transfection indicating proliferation inhibition or cell death induction in Scd1-ablated cells (Figure 2A).
To examine the expression of stem cell related marker genes of pSSCs that indicate proliferation capacity and the inhibition of differentiation, RT-PCR analysis was performed regarding the expression of nestin, Bcrp1/ABCG2, Bmi1, Stat3, Sox2 and Oct3/4.
Colony sizes increased, indicating proliferation, and contained both epithelial and mesenchymal cells.
The number of Col1+YFP+ cells increased towards the spongiosa (Fig. 5E), indicating proliferation and increasing differentiation to osteoblasts.
Perfusion with endothelial cell medium, increased, although not significantly, the numbers of VEGFR-2 + cells, indicating proliferation of these cells.
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