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The diabetes-specific locus of control questionnaire (25), translated into Italian (27), includes the following three domains: internal; powerful others; and chance (scores 6 36); the domain with the highest score indicates locus of control.
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Indeed, a glance at the simple mapping output for our AIL population indicates loci significant by naïve permutation (scale on left axis, trace and line indicating genome-wide significance in red) on Chrs 1, 8, 9, 12, and 16.
However, there was no, or very little, hybridization signal on chromosome Dpo, indicating locus loss or drastic elimination of units.
Then 1 μg of RNA was reverse transcribed into cDNA for analysis by qPCR using primers specific to the indicated locus (using QuantiTect Primer Mix from QIAGEN).
As in line 73, there were only four 35S rDNA signals (some with secondary constrictions) instead of the expected six, indicating locus loss.
Linkage studies in AD may therefore indicate loci harbouring rare variants and nominate candidate genes for re-sequencing.
Originally, the designations "hrp" and "hrc" indicated loci that are required for induction of non-host hypersensitive reaction and for pathogenicity, and individual genes in the loci were given these designations.
We crossed Sk strains to measure recombination frequencies between the indicated loci.
Two of three regions with dominant HLOD > 1.5 overlap with previously indicated loci.
Low F ST outliers indicate loci subject to balancing selection, whereas high outliers suggest adaptative (directional) selection [ 59].
(A ) ChIP-qPCR of 3HA-MITF in 501Mel-CL8 cells at the indicated loci following transfection with siLuc or siBRG1.
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