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Grey shading in the actograms indicates lights off.
The grey shading indicates lights off in a 12 12 LD cycle.
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Red indicates wakefulness, black is REM sleep, and blue indicates light (light blue) to deep (darker blue) sleep.
White box indicates light period, black box indicates dark period, and gray box indicates subjective light period.
RSS score 1 to 4 indicates light sedation, and RSS score > 4 indicates deep sedation.
Asterisk indicates light chain of mouse anti-ratCD2 antibody used in the MACS sorting processing.
A score of 8 16 points corresponds to deep sedation, 17 26 indicates light sedation and 27 40 indicates inadequate sedation [ 4].
Hyb ++ indicates extensive positive in situ hybridization reactions in HP tissue while Hyb + indicates light focal positive reactions and Hyb – indicates no reaction.
The large baseline offset from zero indicates light scattering, which may also be a cause of the baseline sloping (Bassan et al. 2010).
Shaded boxes represent lights-off and yellow boxes indicated lights on.
Overnight, there are several bright 'pulses' of light, indicating lights being switched on and off (d), (e) and (f).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com