Sentence examples for indicates fixed from inspiring English sources

Motif stereotypy is defined as "1 – entropy", thus a stereotypy score of 1 indicates fixed syllable order.

Bold font indicates fixed band absence (≤2.5% frequency of AFLP band) and underlined font fixed band presence (≥97.5%).

The PRL-HeLa cells transiently expressing GFP-ER were treated with E2, 4HT, or EGF for the time indicated, fixed, and DAPI stained.

For γ-H2AX staining, cells were treated as indicated, fixed in 3% formaldehyde and permeabilized in 90% methanol.

In addition, we use an asterisk to indicate fixed or target values, such as a "critical effect size" (M*), target human dose (HD*), or target incidence level (I*).

Briefly, cells were treated as indicated, fixed in 1% paraformaldehyde, washed in PBS three times, incubated in equilibration buffer for 10 min and incubated with terminal deoxynucleotidyl transferase for 60 min at 37 °C.

Proliferation assay was performed using the BrdU CHEMICON® Cell Proliferation Assay Kit (Millipore, 2750) and a microplate reader (Beckman Coulter AD340, Beckman Coulter Inc .. RAW 264.7 cells plated on glass coverslips, were treated as indicated, fixed and stained using DAPI dihydrochloride (InvitrogenTM, D1306).

Wild-type and ncs2Δ elp6Δ cells expressing Hsp104-GFP from the HSP104 chromosomal locus were grown to exponential phase in YPD, treated as indicated, fixed in 3.7% formaldehyde, and imaged on a Zeiss Axio Imager 2 microscope.

Cells on coverslips were treated as indicated, fixed in 3.7% methanol-free formaldehyde (in PBS) for 15 min, followed by permeabilisation in 0.5% Triton X-100 (T-X-100) in PBS for 5 min, and blocked in 1% BSA in 0.1% T-X-100/PBS for 15 min.

For RNAseq studies, cells were fed with media ± harmine at day 2 without polarizing cytokines and harvested at day 4. Sorted CD4+ cells were stimulated as indicated, fixed in 3% paraformaldehyde (Santa Cruz, Dallas, TX), permeabilized in PBS + 2% fetal calf serum + 0.1% Triton X-100 (Sigma Aldrich, St . Louis MO) and stained with anti-CD4-FITC, rabbit anti-NFAT1, APC-donkey anti-rabbit and DAPI.

For analysis of sub-G1 DNA content, LCL8664 cells were treated with cytokine as indicated, fixed, permeabilized, and stained with 20 μg/ml propidium iodide (PI) in PBS containing 3.8 mM sodium citrate and 8 μg/ml RNase A at 37°C for 30 minutes.