Pancreatic cell cultures were seeded into 24-well plates and treated with DMSO or indicated agents.
After 24 h incubation, the cells were washed twice with PBS and incubated with fresh serum-free DMEM containing all the supplements and indicated agents.
Following incubation, the cells were washed twice with phosphate-buffered saline (PBS) and then maintained in a serum-free DMEM including all supplements and indicated agents.
And then, the cells were washed twice with phosphate-buffered saline (PBS) and maintained in a serum-free medium including all supplements and indicated agents.
Cells were plated into the upper chamber in reduced serum DMEM (0.1%) with complete DMEM in the lower chamber and indicated agents added to both chambers.
Following incubation, the cells were washed twice with phosphate-buffered saline (PBS) and then maintained in a serum-free α-MEM including all supplements and indicated agents.
Medium supplemented with 10% serum or indicated agent was added to the outer cup.
Table 2 indicates those agents with large numbers of exposed workers.
Induction of senescence in 3T3-F442A preadipocytes, which are an immortal cell line, indicates these agents can induce preadipocyte senescence independently of replicative history.
The suffix -eer usually indicates an "agent noun", she says, describing a person who performs the action of the verb, in this case operating/acting on an engine.
