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For clotting, bovine thrombin (180 U) (Haematologic Technologies, Inc., Essex Junction, VT, USA) and 10%% CaCl2 (30 μL) were added to the indicated volumes of PRP or PPP preparations to form 5 × 5 × 1 mm (width × length × thickness) membranes.
For the use of 12 mm diameter coverslips, scale up the culture volumes proportionally to the corresponding culture vessel, but use the indicated volumes for blocking solution, antibody solution, click reaction cocktail and DAPI staining solution.
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After induction of lung injury, when the blood pressure and heart rate indicated volume depletion, 6% hydroxyethyl starch solution was added.
As such, the current or dried volume can be smaller than the indicated volume by 8 cubic feet (0.23 cubic meters).
Since staff notation indicates volume, attack, and technical effects in a comparatively clumsy manner, the written page becomes cluttered and unclear.
Mild left hemidiaphragmatic elevation indicates volume loss (a).
The forest area on the left presents a rather strong scattering power indicating volume scattering is prominent.
(C) IVC measuring 2 cm with <10% inspiratory collapse and associated hepatic vein engorgement indicating volume overload.
Relative changes after volume expansion may better indicate volume status [ 27] and, in our opinion, require further study.
PPV has been advocated to indicate volume responsiveness - in part in order to avoid unnecessary fluid loading.
This has been documented by several investigators, thus Muller and colleagues stated that the insufflation pressure must exceed 20 cmH2O for the threshold value of 13% to be validly indicating volume responsiveness [ 22] and De Backer and colleagues argued that the tidal volume must exceed 8 ml/kg for PPV to reliably indicate volume responsiveness at a threshold value of 12% [ 23].
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