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Cells from one well of a six-well plate were then transferred equally into four wells of a 24-well plate and either mock or infected with the indicated virus.
Subsequent serologic evidence indicated virus circulation in dog breeds other than greyhounds, but the virus had not been isolated from affected animals.
Cells were replated, 1 × 106 cmlls/6 cm dish, and transduced (as described for the growth curve assays) with the indicated virus.
The virus infectivity recovered in RSV-infected HEp2 and PMΦ cells was compared by plaque assay which indicated virus titres of 3x10 pfu/ml and 2x10 pfu/ml respectively.
At the times indicated, virus was harvested from the wells and titers were determined on Vero cells (for KOS and hrR3) or on E5 cells (for other recombinant viruses).
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The caller would then warn that those files indicated viruses that could crash the computer or, in at least one case studied by the F.T.C., that the computer could explode.
(F) BALB/c mice were divided into 4 groups randomly and mock infected or infected with the indicated viruses by intramuscular injection (i.m).
Thereafter, cell culture medium was removed and cells were infected with the indicated viruses.
a 10.6.1 transgenic (10.6.1) or wild type (wt) N. benthamiana plants were manually inoculated with the indicated viruses.
(A and B ) IFNAR1 KO mice were injected intraperitoneally with PBS (not infected; NI) or with 10E6 pfu of the indicated viruses.
Together, these results indicate virus can be detected at multiple mucosal surfaces of the respiratory system.
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CEO of Professional Science Editing for Scientists @ prosciediting.com