Cellular uptake assay indicated that Lf-NLC could be internalized into cytoplasm via Lf-receptor mediated endocytosis pathway.
The results clearly indicated that Lf-M-PAEEP-PLLA-NPs exhibited adequate active tumor cell targeting ability through the combination of the active targeting ligand of Lf.
The results indicated that Lf-M-PAEEP-PLLA-NPs exhibited the contrast-enhanced MRI and lower signal intensity compared to M-PAEEP-PLLA-NPs.
The results from the in vitro and in vivo MRI indicated that Lf-M-PAEEP-PLLA-NPs possessed strong, long-lasting, tumor targeting, and enhanced tumor MRI contrast ability.
The zeta potential of both formulations was found to be similar, which indicates that Lf does not markedly affect the cationic surface charges of SLNs.
The results of the studies included in this review indicate that LF stability is dependent on its level of iron-saturation and on the characteristics of the treatment media.
The absorbance spectrum at 465 nm of saturated LF is clearly altered from that of unliganded LF, indicating that LF binds to iron [13].
A previous study indicates that LF's PA binding domain (LFN, the first 263 residues of LF) can increase the rate of PA oligomerization, while soluble monomeric ANTXR2 extracellular domain (msANTXR2) did not appear to influence assembly greatly [16].
Results from our study indicate that LF animals could be experiencing an abnormal decline in cellular growth/proliferation i.e. 21 genes implicated in cellular proliferation inhibition, including FST[ 74], NPPC[ 75], GJA1[ 76], SOX6[ 77], were up-regulated in the LF animals.
