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Typically 0.75 µg of ISG15variant, if indicated supplemented with 0.25 µg E1 and 0.5 µg E2 or, applicable supplemented with 0.75 µg substrate was used in a total volume of 250 µL DNA/CaPO4-transfection mixture.
Surgery is carried out under general anesthesia, if indicated supplemented with an interscalenus plexus block.
The next day, medium was changed to 2i and, if indicated, supplemented with 30 ng/ml GCSF (Peprotech).
If symptoms persist longer than 6 weeks, ultrasound can be performed to rule out cuff rupture if indicated, supplemented by conventional radiographic examination.
The diagnosis of acute pancreatitis is based on clinical features (abdominal pain) and elevation of plasma concentrations of pancreatic enzymes, if indicated supplemented by imaging techniques [ 1- 4].
All cell lines were maintained in a basal medium as indicated, supplemented with 10% fetal bovine serum (FBS) and antibiotics, in a humid atmosphere of 5% CO2 and 95% air at 37 °C.
Similar(53)
X- and Y-axis indicate supplemented glucose concentration and RL7-11 productinity in g/l h, respectively.
Early embryonic hearts were dissected according to the section planes indicated (Supplement, Fig. 1).
Each well contained 200 μl Dulbecco's modified Eagle's medium (DMEM) containing indicated supplements and 1 × 10 cells.
X- and Y-axis indicate supplement glucose concentration and carbon yield in RLmol/Glumol, respectively.
Results indicated that supplemented media accelerated collagen formation (∼185% increase in collagen mass/MSC compared to standard media), as well as increasing collagen mass production from 3.90 to 4.43 pg/cell/week from 3 to 6 weeks.
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