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Injected embryos were treated with 10mM of dexamethasone (Dex) at indicated stages.
Integrated fluorescence intensity of DR5 GFP signal of whole embryos at the indicated stages.
Total RNA was prepared at the indicated stages using TRIZOL reagent (Invitrogen, Carlsbad, CA).
Embryos were grown to the indicated stages and processed essentially as described previously.
Selected regulated genes with established or potential roles in angiogenesis between indicated stages are listed in tables 1 3 (for complete list of regulated genes, see Supplementary table S1).
Intestinal fragments were isolated from the anterior part of the small intestine just after the bile duct junction from tadpoles at indicated stages as well as premetamorphic tadpoles after 10 nM T3-treatment and fixed in MEMFA followed by cryo-sectioning.
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While the riders' heart rates indicated stage fright in the live performance, the horses showed about the same levels of stress in both situations.
Estimated GFR (eGFR) of 60 89 mL/mindicated indicated stage 2 CKD, eGFR of 30 59 mL/min/1.73 m indicated stage 3 CKD, and eGFR of 15 29 mL/mindicated indicated stage 4 CKD.
When indicated, stage 54 tadpoles were treated with 10 nM T3 for 0-7 dats at 18°C.
In addition, developmental analysis of Hoxa gene methylation indicated stage-specific methylation differences.
In patient 15 it was contrariwise: CT returned stage IIC disease (N0) whereas SSTR-PET indicated stage IIIB (N1b); targeted lymph node extirpation confirmed N1a disease.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com