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If unacceptable risk is indicated, restrictions on the use are made [6, 8 10] 1107/2009/EC).
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Isolation indices were generally significantly greater than zero, indicating restrictions in potential gene flow.
However, as has been shown, the historical development proceeds differently:N+ → Q+ → R+ → R → C → H, where the plus sign indicates restriction to positive elements.
Underlined sequence (tccgga) indicates restriction enzyme site by ACC III.
Tnos: terminator of nopaline synthetase; HYG R): hygromycin-resistence gene; 35S: cauliflower mosaic virus (CaMV) 35S promoter; pSH4: SH4 promoter; XbaI and BglII indicate restriction sites; gusA: β-glucuronidase (GUS) gene; RB: right border; LB: left border.
The resulting PCR products were digested with the indicate restriction endonucleases and sequentially ligated into pET21b.
Primer sequences used in this work (the underlined sequences indicate restriction sites, the restriction site of BstP I is inside the PCR product) are listed in Table 3. Pdr5p-GFP protein fusions were constructed as previous described[55].
Underlines indicate restriction enzyme sites.
The underlined sequences indicate restriction enzymes used.
Arrowheads indicate restriction fragments containing the chromosomal UMPS sequence.
*Sequences in boldface indicate restriction sites for BamH1 and XhoI.
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