Sentence examples for indicated restriction from inspiring English sources

Subsequently, a contiguous sequence extended to 3,200 bp (glu) was obtained and confirmed by PCR amplification with primers R.pGWf/R.pGWr.> -wrap-foot> The primers are denoted as follows: the start codon and stop codon are underlined; the cons'ervative codons are boxed; the dotted line indicated restriction enzyme sites.

About 3 5 μg of circular plasmids were digested by indicated restriction enzymes.

The deduced β-xylosidase gene was ligated at the indicated restriction site.

RT-PCR samples were then treated with and without the indicated restriction enzymes obtained from New England Biolabs.

For PFGE analysis, genomic DNA was embedded in agarose using the CHEF Genomic DNA Plug Kit (Bio-Rad), digested with the indicated restriction enzymes, and electrophoresed in 1% agarose using the CHEF Mapper XA system (Bio-Rad).

10 20 µg genomic DNA from the tails of mice was digested with the indicated restriction enzymes and analyzed by Southern blot using a-P32-dCTP labeled random-primed DNA probes (Stratagene Prime-It II kit).

If unacceptable risk is indicated, restrictions on the use are made [6, 8 10] 1107/2009/EC).

However, as has been shown, the historical development proceeds differently:N+ → Q+ → R+ → R → C → H, where the plus sign indicates restriction to positive elements.

Underlined sequence (tccgga) indicates restriction enzyme site by ACC III.

The (3)He D values measured perpendicular to the length of the trachea were approximately a factor of two less, indicating restriction to diffusion.

Tnos: terminator of nopaline synthetase; HYG R): hygromycin-resistence gene; 35S: cauliflower mosaic virus (CaMV) 35S promoter; pSH4: SH4 promoter; XbaI and BglII indicate restriction sites; gusA: β-glucuronidase (GUS) gene; RB: right border; LB: left border.