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P. luminescens carrying the indicated reporter gene plasmid was grown in complex medium and in complex medium containing insect homogenate (in vitro).
Indicated reporter strains were grown overnight in M9 minimal medium containing 0.2% glucose, 0.05% casamino acids, and 50 μg/ml of kanamycin with shaking at 37°C.
K562 cells were cotransfected with 1 µg of indicated reporter (CD71 3'UTR or mutCD71 3'UTR), 50 ng renilla luciferase construct and 2 µg of indicated microRNA expression constructs, all combined with Lipofectamine 2000 (Invitrogen).
The next day, cells were transfected with the indicated reporter plasmids using FuGENE6 transfection reagent (Roche).
Transient transfections were conducted using PolyFect or JetPEI reagents of cell cultures in 24-well culture plates with the indicated reporter plasmids, then cotransfected with mammalian expression vectors.
U2OS cells were plated at a density of approximately 50% in 100 mm tissue culture plates and transfected in triplicate with 5 µg of both the indicated reporter and expression constructs as specified above.
Similar(43)
Arrows show areas of upd3.3LacZ expression in areas with epithelial organization; arrowheads indicate reporter activation in regions with disrupted architecture.
We have a video where —— " Mr. Jonze interrupted him and indicated the reporter in the room.
The list included not only several former and current staffers, but also indicated the reporter called Kirk's 79-year-old mother and his sister multipe times. .
(1) 'Present' indicated that reporter gene expression was observed in that tissue.
(2) 'Not detected' indicated that reporter gene expression was not observed.
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CEO of Professional Science Editing for Scientists @ prosciediting.com