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The next day, fresh medium or medium containing the indicated quantities of drug was applied to the dishes.
Fluorescently labelled DNA substrate (3 nM) was incubated at 30 °C with the indicated quantities of Srs2 in buffer E (30 mM Tris pH 7.5, 1 mM DTT, 0.1 mg/ml BSA and 100 mM KCl) for 10 min.
Fluorescently labelled DNA substrate (6 nM) was incubated at 30 °C with the indicated quantities of Srs2ΔC, Rad5 or Mph1 in buffer D (25 mM Tris pH 7.5, 1 mM DTT, 0.1 mg/ml BSA, 50 mM KCl, 7.5 mM creatine phosphate, 11.25 μg/ml creatine kinase, 2.5 mM MgCl2 and 2.5 mM ATP) for 15 min.
Fluorescently labelled DNA substrate (3 nM) was incubated at 30 °C with the indicated quantities of Srs2 in buffer H (30 mM Tris pH 7.5, 1 mM DTT, 0.1 mg/ml BSA, 100 mM KCl, 20 mM creatine phosphate, 20 μg/ml creatine kinase, 2.4 mM MgCl2, and 2 mM ATP) for 10 min.
Similar(56)
Note. 1, 2, or 3 indicates quantities of components contributed to cumulative values.
Note that in that case, at equilibrium, the relation is well known [ 5] (2) where lower case indicates quantities of corresponding species.
Patients were asked to indicate quantity and average weekly frequency of consumption for the period under investigation.
The characterization results indicated that quantities of 517 phase magnesium oxysulfate whiskers were detected in the modified MOS cement.
These findings indicated lower quantities of gas vesicle formation in strains expressing smaller fragments of GvpC and higher quantities in strains expressing larger GvpC fragments or the complete GvpC protein.
In seconds, it spits out a spectrograph that indicates the quantities of hundreds of different molecules within the tissue.
Investigations at farm level compiled with the previous studies (ORMVA/SM 2009; Heck and El Harrak 2010), Table 4 indicate the quantities of potential macronutrients in the organic wastes.
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