Cells were nucleofected with the indicated siRNAs and cultured for indicated periods of time.
Absorbance at 420 nm was measured at indicated periods with a proper reagent blank.
hBMEC cells were treated with sublytic concentrations of suilysin protein for indicated periods after pretreatment regimens.
Besides, hygrothermal monitoring indicated periods of high humidity values and the presence of condensates on the surfaces of the VIPs.
(C) MEFs from wild type, CARMA3−/− (Card10−/−), and Bcl10−/− embryos were isolated and stimulated with doxorubicin (1.5 μg/mL) for indicated periods.
hBMEC cells were serum-starved and then treated with (A and B) S. suis supernatant or (C and D) suilysin protein at sublytic concentrations for indicated periods.
Cells were harvested at the indicated periods of time and nuclear extracts were performed.
Cells were stimulated as described above for the indicated periods of time.
For this purpose, fibroblasts expressing ectopic Nab2 were incubated with TGF-β for indicated periods.
The transfected cells (72 h post-transfection) were then treated with S100A14 for indicated periods.
Protein lysates were harvested from cells incubated in normoxia or hypoxia for the indicated periods.
