Sentence examples for indicated overnight from inspiring English sources

I totally accept that what I say might not be popular – clearly, as the polls have indicated overnight".

These starter cultures were incubated (37 °C and 5%% CO2, unless otherwise indicated) overnight before being diluted to inoculate cultures in 96-well plates at an optical density (OD, measured at 630 nm) of 0.008 for 160 µL (OD of ≈0.02 for 1 cm path length).

Pre-cleared samples were then incubated with 50 μg of specific antibodies as indicated overnight at 4°C.

After permeabilization with 0.3% Triton X-100 and 5% goat serum containing PBS, cells were incubated with primary antibody as indicated overnight at 4°C.

Cells were treated with IFNγ (100 IU/ml, PeproTech), TNFα (100 IU/ml, R & D System), and Lipopolysaccharide (LPS, 1 μg/ml, Sigma-Aldrich) as indicated overnight.

To avoid nonspecific staining, the sections were incubated in blocking solution (5% BSA, Sigma, Germany) for 1 h at room temperature, followed by treatment with rabbit polyclonal anti-GLP1R antibody (1 100, Abcam lnc., UK), or rabbit polyclonal anti-MAPK10 antibody (1 100, Abcam Inc., UK), where indicated, overnight at 4°C.

Military officials indicated tonight that new operations in the hunt for him were under way.

That is, Scott indicated tonight, unless his doubts about his team's courage persist.

But Ajax indicated last night that they were confident he was happy to stay.

The Navy may be pulling out of Vieques over the next two years, as the White House indicated last night.

PETR SVOBODA, his agent, indicated last night that Rucinsky was close to re-signing on the Rangers' terms.