The same number of DAC-positive neurons was detected in mutant and control embryos (Supplementary Figure S2), indicating no defects in their production.
Results from Day 1 indicate no defects in AD or AD + J147 in the ability to swim or see as both have similar escape latency.
Phagocytosis by BMDMs and bone marrow-resident monocytes was unaffected by age, indicating no defect in phagocytosis in the myeloid progenitors of old mice in our model.
After treatment with 12- O-tetradecanoylphorbol-13-acetete (TPA) (1000 ng ml−1) for 1 h, the membrane fractions of conventional PKCs, PKC α and PKC γ increased in all cells, indicating no defect of the PKC pathway in transfectants overexpressing gelsolin as well as the neo-control clones and parental PC10 cells.
For quantitative analyses of the parallel growth of motoneuron axons with the DA, 'complete' indicates the no defects in the motoneuron axons beneath the DA above yolk tubes.
After culture in the presence of mouse recombinant M-CSF and mouse recombinant RANK-L for 1 week on plastic dishes, TRAP staining showed that the number of TRAP positive multinucleated cells is not different between genotypes, and indicated no differentiation defect ex vivo (Fig. 6).
DOI: http://dx.doi.org/10.7554/eLife.02792.016 All strains also showed similar growth rates in rich media and no significant differences in sensitivity to hydroxyurea, methyl methanesulfonate (MMS), bleomycin, 4-nitroquinoline 1-oxide (4NQO), cycloheximide, and rapamycin, indicating no major defects with DNA replication or repair, protein synthesis, or the TOR signaling pathways.
The percentage of TCR βhigh thymocytes was also comparable in CBAP-deficient and WT mice (Supplementary Figure S1c), indicating no obvious defect in TCR rearrangement and maturation.
However, this age-related impairment in phagocytosis was not observed in bone marrow-derived macrophages (BMDMs) or monocytes isolated directly from bone marrow of old mice, indicating no intrinsic defect in phagocytosis in macrophage progenitors in this model.
The high-energy Raman modes remain unchanged, indicating that no defects are introduced when stretching.
