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Originally, the designations "hrp" and "hrc" indicated loci that are required for induction of non-host hypersensitive reaction and for pathogenicity, and individual genes in the loci were given these designations.
We crossed Sk strains to measure recombination frequencies between the indicated loci.
Two of three regions with dominant HLOD > 1.5 overlap with previously indicated loci.
(A ) ChIP-qPCR of 3HA-MITF in 501Mel-CL8 cells at the indicated loci following transfection with siLuc or siBRG1.
A set of 16 files, one for each chromosome, contains the SGA-GD between the indicated loci.
bAverage G + C content is presented for single locus or as an average value of the indicated loci cluster or operon.
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Then 1 μg of RNA was reverse transcribed into cDNA for analysis by qPCR using primers specific to the indicated locus (using QuantiTect Primer Mix from QIAGEN).
Linkage studies in AD may therefore indicate loci harbouring rare variants and nominate candidate genes for re-sequencing.
Indeed, a glance at the simple mapping output for our AIL population indicates loci significant by naïve permutation (scale on left axis, trace and line indicating genome-wide significance in red) on Chrs 1, 8, 9, 12, and 16.
Low F ST outliers indicate loci subject to balancing selection, whereas high outliers suggest adaptative (directional) selection [ 59].
Ten Ah SSR primer pairs out of 82 analyzed amplified more than one locus in tetraploid accessions of Arachis section indicating loci duplication.
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