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Moreover, fluorescently-tagged human recombinant PLCζ indicated localisation to discrete regions within the cell cytoplasm when expressed in HEK293T cells, adjacent to the nuclear envelope, possibly representing a specific organelle sub-type [ 74].
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The results indicate localisation of NO3− within the rhizosphere of biochar-amended soils which has implications for NO3− loss and improved nitrogen use efficiency.
The results of the trafficking studies with activated V γ9V δ2 T cells reported here indicate localisation of V γ9V δ2 T cells at a large tumour site from as early as 1 h after infusion, with maximal activity at 4 h and persistence for at least 48 h.
The green spots indicated the localisation of micrometre-sized UHMWPE wear particles within the cells, which were also localised around the periphery of the nuclei (Fig. 11b).
These data appear to strongly indicate the localisation of ADPM01 within the endoplasmic reticulum; however, due to their close perinuclear proximity, additional localisation within the Golgi apparatus cannot be ruled out.
- The data on the exact localisation of plasmepsin V, while not exactly excluding the possibility suggested in this review (page 2), could as well indicate a localisation within the ER.
In addition, there are several intensely fluorescent pockets that indicate intranuclear localisation.
An Fn/c value of 1 indicates diffuse localisation, with an equivalent amount of protein in both the cytoplasm and the nucleus.
Furthermore, siRNA containing particles colocolized (or resided in a close proximity) with astrocytic GFAP cytoskeleton and cells nuclei indicating intracellular localisation.
This colocalisation of IQGAP1 and F-actin becomes evident in the enlarged micrographs and the merged image shown in Figure 4B, in which the yellow colour indicates similar localisation of the two proteins.
As mutations that abolished cytoplasmic trafficking diminished anchorage-dependent growth and failed to protect against UV-induced apoptosis, indicating cytoplasmic localisation of PDK-1 plays pivotal roles in signalling (Lim et al, 2003).
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