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The infected cells were then incubated in gentamicin media for the indicated length of time and assayed as described below.
Briefly, mid-log cell cultures were grown on appropriate medium, and treated or not treated with 0.5 M KCl for the indicated length of time.
Where indicated, medium was replaced with medium containing PP1 (SYNTHOSE Ltd), rapamycin (ICN Biochemical Inc), AICAR (Toronto Research Chemicals Inc) or compound C (Merck), 48 h after initial plating, and cells were further incubated for the indicated length of time.
Left: representative traces of fEPSPs, evoked by stimulation for the indicated length of time.
In brief, cells were treated with or without bortezomib for the indicated length of time.
Approximately 2 × 10/ml MDA-MB-231 cells were transfected with ANT1 and cultured for the indicated length of time.
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Cells were seeded on to collagen-coated (0.5, 1, 2 mg/mL) or uncoated non-tissue culture plastic for indicated lengths of time.
iFDM cells from patient P1, P2, and normal NHDF-2 fibroblasts were allowed to differentiate for indicated lengths of time to form myotubes.
For steroid treatment, cells were first cultured in phenol-free DMEM (GIBCO) containing 10% double charcoal-stripped FBS (Bioind, Kibbutz Beit Haemek, Israel) for 72 h and then incubated with 10−8 M 17β-estradiol (E2) (Sigma, St Louis, MO, USA) or 10−6 M 4-Hydroxytamoxifen (4-OHT) (Sigma) dissolved in ethanol, or with ethanol only (as a vehicle control) for indicated lengths of time.
Year of arrival, indicating length of time in the resettlement country, is recommended for inclusion in routine health datasets, but has yet to be universally adopted [ 1, 2].
Age was an independent predictor for HBV infection, as reported in the literature, 19 indicating length of time for viral exposures and initiation of sexual activity.
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