Membranes were incubated with the specific antibodies as indicated in the figures; the secondary antibodies used were horseradish peroxidase-conjugated anti-mouse and anti-rabbit antibodies.
Sections were reacted with the primary antisera indicated in the Figures, and then secondary antisera conjugated with R-phycoerythrin or Alexafluor 546 (Molecular Probes/Life Technologies, Carlsbad, California).
As indicated in the figure, both primary and secondary industries exhibited negative industrial composition effects throughout the 15-year period from 1990 to 2005, whereas tertiary industries exhibited an overall positive effect throughout this period.
Cortical cultures were lysed in RIPA buffer as described previously, and equal amounts of protein (25 50 μg) were analyzed by immunoblotting, using the primary and secondary antibodies indicated in the Supplementary Information.
Secondary reconstruction is indicated in the presence of reasonable ankle function and even in the presence of arthritic changes [7, 9].
Alterations in the spectrum of circular dichroism indicated changes in the secondary protein structure.
The Scandinavian Glutamine Trial indicated in secondary outcomes of only patients treated per protocol that iv glutamine supplementation may improve outcome during ICU stay [ 12].
53 Furthermore, risperidone is indicated in dementia and secondary psychoses.
Thus, it indicated that the secondary vegetation in the study area had been damaged seriously, and the construction of artificial vegetation lagged behind, so there was a potential danger in ecological safety, which should be concerned and precautioned.
Circular dichroism (CD) spectra on both metallated and apo proteins indicated that the secondary structure present in WT SOD1 was fully conserved in all of the mutants.
