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RuCl3 · 3H2O and Ru acac 3 are catalysts for the electrooxidation of water and the role of electrogenerated radicals in the process is indicated, in support of previous work.
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The determination of the platelet surface expression of αIIbβ3 integrin was performed by flow cytometry using platelet-rich plasma (PRP) aliquots and specific antibodies as indicated in Supporting Information S1.
Primers used are indicated in Supporting Information, Table S1.
The primers used are indicated in Supporting Information Table SI.
Selected genes were validated by qRT-PCR using the primers indicated in Supporting Information Table S2.
Conditions for PCR protocols are indicated in Supporting Information Table S3.
The step-by-step procedure of integrating GBS tags into the POPSEQ assembly is indicated in Supporting Information, Figure S1.
Squalenoyl nanocomposites were prepared using the nanoprecipitation method after optimization as indicated in Supporting Information and the best formulation was used for further in vitro and in vivo studies.
As indicated in Supporting Figure 2, when a read was separated into two contigs, the coordinate of the read in each contig was shown after the read name, followed by the contig number with which this read was linked.
Promoters from 11 of the library members were moved back into the pACSA_Pcpt_YFP construct using in vitro recombination assembly with overlapping primers (Gibson 2011) using the primer pairs indicated in Supporting Information Table 3.
These constructs were made using a two piece Gibson Assembly using primer pairs indicated in Supporting Information Table 3. Transcription start sites were mapped using the 5′ RACE System for Rapid Amplification of cDNA Ends, Version 2.0 (Invitrogen).
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