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Antibodies were diluted as indicated in Material and Methods S1.
The positive HER2-ERBB2 cut-off criserindicateddinated in Material and Methods.
The red fluorescence intensity associated to the entire cells was quantified as indicated in Material and Methods.
To verify the endothelial potential of sorted cells, the CD133+CXCR4+CD34- cells from normal and dystrophic blood were cultured in the presence of endothelial medium (End) as indicated in Material and Methods.
These spots were excised from preparative-gels, and subjected to enzymatic digestion (as indicated in Material and Methods).
Proteins selected were subjected to enzymatic digestion (as indicated in material and methods), the resulting peptide fragments were analyzed using the complementary mass spectrometry methods MALDI-TOF-MS and LC-ESI-MS/MS.
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Cell density was estimated at the indicated times by crystal violet assay and quantified as indicated in Materials and Methods.
The antigens were designed as indicated in materials and methods.
After 45 min incubation, cells were lysed and cAMP production was determined by ELISA as indicated in "Materials and methods".
Hep3B cells were exposed to hypoxic stress 4 hours post transfection as indicated in Materials and Methods.
Antibodies (indicated in 'Materials') were added in TBS-T containing 2.5% skimmed milk and the blots were washed with TBST (TBS +0.1% Tween 20).
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