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For microscopic analysis, cells were seeded into eight-well Millicell slides (Millipore) and treated as indicated in duplicate wells.
The cells were then rinsed with DMEM, reseeded at the cell number indicated in duplicate, and incubated for 10 to 14 days until colonies formed.
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The duplicated Affymetrix IDs are indicated in the 'Duplicated' section of the results page.
Experiments were performed three times in duplicate (otherwise indicated in the legends).
Assays were usually performed at least three times in duplicate, otherwise indicated in the legends, and one representative experiment is shown.
Duplicated pairs indicated in parentheses " indicate that a single CNE was localized within duplicated pseudolinkage group within a male mapping panel.
In both analyses, results were generally consistent with a single gene being detected in the expressed gene sets across diverse angiosperms, though duplicates were indicated in some species, particularly in recent polyploid taxa [ 49].
Experiments were performed in duplicate or triplicate and repeated as indicated in the figure legends.
Each qRT-PCR was performed in technical duplicate and on biological replicates as indicated in the figure legends.
Primary murine bone marrow colonies were grown in MethoCult GM Stemm cell Technologies, Vancouver, BC, Canada) supplemented with the indicated drug dosage in duplicate and counted after 7 days in culture.
Therefore, the WGD in paddlefish is indicated by large, duplicate gene clusters that are likely located at different chromosomal loci.
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