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The resulting psychometric curves indicated hit rates between 0.45 and 0.71 during presentation of the strongest stimulus change and false alarm rates between 0.29 and 0.43 during catch trials (with n = 49 316 trials per stimulus).
(C ) The number of precocious 2° islets was quantified following treatment with the indicated Hit I compounds from 3 to 5 dpf.
(M ) The number of precocious 2° islets was quantified following treatment with the indicated Hit I compounds from 3 to 5 dpf.
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Results of the 25 real-time feedback sessions are provided in Figure 7. Panels A C indicate hit rate, mean movement time, and mean endpoint error (measured as the difference between the formant space position at the end of the trial and the target formant position for that trial) as a function of block number, averaged across all 25 sessions.
1Rank of pocket-centers within 4 Å of the considered ligand (brackets indicate hits exceeding the 4 Å criterion).
Asterisk indicates hits only present in normalized GS-FLX reads.
Rounded rectangles indicate helix elements, purple circles indicate hits corresponding to < 30 Å pairwise distance in the crystal structure, pink circles indicate hits corresponding to > 30 Å pairwise distance in the crystal structure, and black circles indicate MOHCA-gel 'spectator hits', which appear due to lack of rigorous background subtraction (Das et al., 2008 ; Kim et al., 2011 ).
In (C ) and (D ), rounded rectangles indicate helix elements with colors matching helices in (E – F ), purple circles indicate hits corresponding to < 30 Å pairwise distance in the crystal structure, and pink circles indicate hits corresponding to > 30 Å pairwise distance in the crystal structure.
The antifungal agent, ketoconazole, is known to bind to CYP126 in a typical azole heme type-II coordination mode by using heme absorbance shift assays (Kd=1.4 μ m; K. J. McLean et al., unpublished results), and ketoconazole was used here in displacement experiments to indicate hits that might bind within the CYP126 active site (displacement in CPMG only).
Analysis of variance indicated that HIT-6 scores discriminated across both types of criterion groups (P<0.001), across studies and time points.
The compound was screened at the DC120 concentration of 0.1 and 1 μM, and the results for primary screening were reported as %Ctrl where lower numbers indicated stronger hits according to the DiscoveRx protocol.
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