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Where indicated, extracts were dialyzed against water in a 1 kDa membrane for 4 hours.
Where indicated, extracts were prepared in the presence of protein phosphatase inhibitors to preserve serine/threonine phosphorylation events and phosphorylation-dependent interactions [ 34].
Where indicated, extracts were prepared in the presence of protein phosphatase inhibitors (1 μM microcystin-LR, 50 mM NaF and 10 mM sodium orthovanadate) to preserve phosphorylation-dependent interactions [ 34].
Spores were incubated anaerobically with the indicated extracts for 30 min. at 37°C, at which point dilutions were made, spread onto individual BHIS and BHIS + TA plates, and colonies enumerated after overnight growth.
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When indicated, extract was preincubated with the inhibitors recombinant GAT165 314, PAK-PBD, and waspΔvca, as described (Koronakis et al., 2011).
The abbreviation 'Acad' indicates extracts from academic researchers.
Blue and gray segments indicate extracted data and other data, respectively.
The green points in Figs. 7(b e) indicate extracted the elliptic points, and the red ellipses are the fitted ellipses to those extracted points.
At the end of experiment (120 min), the preparation with V. flavicans extract was 97% (±10%) responsive, indicating extract efficacy.
The analysis of five-fold independent DNA extracts indicated low inter-extract variability, with a coefficient of variation ranging from 0.52 to 6.08% for Bacteroidetes, from 0.98 to 4.81% for Firmicutes, and from 0.64 to 2.67% for Lactobacillus (Table 3).
Where indicated, mitochondrial extracts were crosslinked in the presence of the zero-length crosslinker EDC11.
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