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Finally, participants indicated expressions of interest in and/or commitment to specific priorities.
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Purified GST-fusion constructs bound to Glutathione agarose beads were incubated with H4 cell lysate expressing indicated expression constructs.
Immunohistochemistry indicated expression of OX-R1 in both species in neurons that also expressed CCKA and leptin receptors.
Another transcription study using mixed sugar medium also indicated expression level of GCY1 increased even in glucose fermentation phase (Alff-Tuomala et al. 2016).
Twelve hours later, cells were transiently transfected with a total of 10 µg of empty vector or indicated expression plasmids using Lipofectamine 2000 (Invitrogen, Cat. No. 11668-027).
GFP fluorescence of the reporter strain indicated expression of the corresponding gene.
Where indicated, expression of two selected proteins in ∼200 cells in randomly specified areas was quantified.
Moreover, RT-PCR analysis indicated expression of Nestin, Sox1, Sox2, Sox3 and Blpb in NPCs (Figure 1D).
Our analysis indicated expression increase in over 30 genes classified as belonging to this gene family.
The results indicated expression of HSP27 in MHCC97H cells was efficiently inhibited.
For other molecules indicated, expression was not significantly different between normal and tumour samples.
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