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GFP fluorescence of the reporter strain indicated expression of the corresponding gene.
Previous reporter gene analysis with a <10 Kbp reporter construct indicated expression of the gene in ASE, as expected, but also in several other neuron types [22].
GFP fluorescence of different E. coli strains (wild type or single gene knockout mutant strains) carrying reporter plasmids pgadX-gfp or puspE-gfp indicated expression of the gene gadX or uspE in these strains, respectively, under the tested experimental conditions.
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Green boxes indicate expression of the pair on the left (rows) in the indicated neurons or region listed above the grid (columns).
From each neuronal culture, approximately fifteen cells were selected based on positive GFP expression (indicating expression of the cholinergic Cha-GAL4 driver).
MFI ratio values greater than 1 indicate expression of the molecule on the cell surface.
Most of the predicted genes could at least partly be covered by EST sequences indicating expression of the genes.
Because the presence of cDNA or EST clones indicates expression of the corresponding genes, we performed searches against the cDNA/EST rice databases.
Quantification was achieved using the ΔCt method which indicates expression of the target gene relative to an endogenous reference (GAPDH; Hs99999905_m1).
Polyclonal anti-Avd indicated expression of both the Avd-pIII fusion (upper arrow, ~38 kDa) and the free Avd (lower arrow, ~15 kDa).
The results indicated expression of HSP27 in MHCC97H cells was efficiently inhibited.
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