Purified GST-fusion constructs bound to Glutathione agarose beads were incubated with H4 cell lysate expressing indicated expression constructs.
Immunohistochemistry indicated expression of OX-R1 in both species in neurons that also expressed CCKA and leptin receptors.
Among these, 85 genes (62.04%) were expressed based on the EST data; while the microarray data indicated expression evidence for 60 genes (43.79%) (Additional file 7).
Twelve hours later, cells were transiently transfected with a total of 10 µg of empty vector or indicated expression plasmids using Lipofectamine 2000 (Invitrogen, Cat. No. 11668-027).
Another transcription study using mixed sugar medium also indicated expression level of GCY1 increased even in glucose fermentation phase (Alff-Tuomala et al. 2016).
Recent reports have indicated expression of H1 and H2 receptors in germinal and peritubular cells of the testis, as well as in macrophages and Leydig cells.
HBsAg, anti-HBs, HBeAg, anti-HBe, and anti-HBc detection indicated expression and replication of this HBV genome in mice, similar to other HBV isolates.
GFP fluorescence of the reporter strain indicated expression of the corresponding gene.
Where indicated, expression of two selected proteins in ∼200 cells in randomly specified areas was quantified.
Moreover, RT-PCR analysis indicated expression of Nestin, Sox1, Sox2, Sox3 and Blpb in NPCs (Figure 1D).
Previous reporter gene analysis with a <10 Kbp reporter construct indicated expression of the gene in ASE, as expected, but also in several other neuron types [22].
