Exact(2)
nlg-1 (C40C9.5c), nrx-1α (C29A12.4a), nrx-1β (C29A12.4f), frm-3 (H05G16.1), lin-2 (F17E5.1a), and unc-49B (T21C12.1b) cDNAs were cloned from a cDNA library using primers corresponding to the predicted start and stop codons oF17E5.1adicandd EST.
The X. laevis NabKin cDNA sequence corresponding to amino acids 1 705 (IMAGE:7010529/IMAGp998K0714717Q, SourceBioScience) and 871 1541 (IMAGE: 4040452/IMAGp998E139296Q, SourceBioScience) was then determined by direct sequencing of indicated EST clones.
Similar(58)
As previously indicated, EST-based comparisons between soybean and disparate genomes have been used to explore evolutionary distance and synteny [ 18].
Arrows below the schematic representation indicate EST groups anchored to the element.
Not all of the cfNRPD entries are yet represented in the EST databases; the hpDB allowed us to identify highly conserved proteins and searching the cfESTDB directly indicates ESTs that may be translated.
trichocarpa, P. tremula, and a P. tremula x P. tremuloides cross), the two complete LKR/SDH genes have distinctive 3' UTRs with matching ESTs (Additional File 10) that indicates ESTs originating from both genes (gene 1 matches ESTs CV242527, DV242515, DB907693, DB900296; gene 2 matches ESTs DB899866, CK105181, CK095239, BI126461).
First, RNAi duplexes that effectively targeted NGLY1 expression were sought, but searches in silico indicated several EST sequences potentially arising from alternative transcripts.
Gene expression frequencies are also indicated in EST data.
It is indicated that EST sequencing is an efficient method for gene discovery and expression level analysis.
RT-qPCR analysis of eight genes indicated that EST frequency in the MC library, constructed using the Cap-Trapper method, does not correspond to relative gene expression.
As indicated by EST data, poly(A) stretches were implicated in the creation of complete stop codons from incomplete T codons [ 31] in ND3, ND6, ND4, and ND5.
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