We induced experimental colitis in C57BL/6 mice by adding 3.5% DSS to the drinking water for indicated days.
During the period of DSS treatment, the weight loss was noticed after DSS administration under the indicated days.
Groups of mice (n = 6 mice/group) were treated with hyperosmotic medium (610 mOsm) prepared by dissolving mannitol (0.3 M) in phosphate buffered saline (PBS) for indicated days.
All treated mice were sacrificed and examined at indicated days.
Thus, GPX1 protein level was measured at indicated days of POLGdn induction by western blot assay.
Total RNA was isolated from embryo and endosperm on indicated days after pollination (DAP).
The secreted HBeAg and the 3.5 kb HBV RNA were determined on indicated days after infection.
Secreted viral antigens and intracellular viral replication intermediates cccDNA and/or RNAs were examined on indicated days after infection.
ALT blood level of control (WT) and YapCKOAlb-Cre (KO) mice measured at indicated days after CCl4 treatment.
The secreted antigens HBsAg and HBeAg, and the intracellular HBV RNAs were determined on indicated days after infection.
mRNA was prepared from replicate (>6) cultures on indicated days from undifferentiated hESCs (day 3), d0, d5, d15, d22, and d25 cells.
