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Where indicated, day 4 tumor spheroids were treated with 17-AAG (Invivogen, San Diego, CA, USA), or PI-103 (Charnwood Molecular Ltd., Loughborough, UK).
On the 11th day or indicated day following treatment, cells were stained for the senescence-associated β-gal (SA-β-gal) marker [ 46] and DNA synthesis was measured over a 24 h interval using a BrdU labeling kit (Roche Diagnostics).
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The Scottish authorities indicated Thursday that they would decide next week whether to free him.
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But the exchange indicated Thursday that it could revisit that decision based on fresh evidence.
A longtime friend of Walsh's indicated Monday that he thought Walsh would be retained.
American officials indicated Wednesday that the White House was still opposed to providing weapons.
Mr. Shulman indicated Tuesday that he had been aware of those findings.
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