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All entry experiments were ended at the times indicated by the addition of 4% paraformaldehyde (PFM), with the exception of those involving transferrin which were ended using 4% PFM with 0.25% glutaraldehyde.
Exenatide intensification was indicated by the addition of a new glucose-lowering medication while glargine intensification was indicated by the addition of a new glucose-lowering medication or by a dose increase of at least 100%.
The expression of Syk-EGFP was induced where indicated by the addition of 1 μg/mL doxycycline for 24 h.
Splice variants should be treated like other paralogs, but can optionally be indicated by the addition of 's' before a paralog number where desirable for clarity, as in macroH2A.1.s1 and macroH2A.1.s2.
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A female character was indicated by the simple addition of a kerchief on the head.
(B ) Immunoblots showing degradation of mCherry-HvyA (indicated by the arrowhead) after addition of chloramphenicol (2 μg/ml) to stop protein synthesis.
This contention has been countered by studies which have indicated that the addition of pyrophosphate to a fluoride dentifrice does not interfere with the anticaries effects of fluoride, mainly based on in vitro and animal studies [ 22].
Reactions were stopped at the times indicated by addition of 500 ng of HindIII-digested λ DNA and sucrose to 5% (w/v) before electrophoresis on 0.2 × Tris-Borate EDTA (TBE), 5% native polyacrylamide gels.
The calcium signal in some neurons (as indicated) is suppressed by the addition of odor (see methods and materials section).
The presence of tannins was indicated by the formation of a precipitate after the addition of 1% gelatin to the filtrate.
Hence, our approach allowed for quantification of both autolysosome formation and degradation in a highly specific manner, indicated by the block of GFP/mCherry turnover by addition of Baf.
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