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(B) BMDCs from wt or DC pten -/- animals were stimulated as indicated and analyzed by western blot for the indicated proteins.
Whole blood samples were collected from 3 volunteers and irradiated at the doses indicated and analyzed with the CLPA assay.
Aliquots were removed at distinct time points as indicated and analyzed by LC-MS/MS with detection of the compound at 254 nm.
CD4 T cells from four HIV-infected donors were isolated by negative selection, cultured overnight, and then stimulated with gp120 (IIIb), and other apoptosis inducing agents as indicated, and analyzed for p24 production 18 hours later (Figure 1C).
Cells were lysed on ice, treated with RNase A as indicated and analyzed by northern blot.
For HBV infection, secreted HBeAg was collected every 2 days as indicated and analyzed by ELISA.
Blood cultures were drawn when clinically indicated and were analyzed locally.
This analysis excluded localized, stage I cancers for which chemotherapy is not indicated and then analyzed colon cancer care among 2,319 women with stage II to IV disease [ 23, 24].
Cross-linked protein samples were precipitated by Streptavidin Dynal beads followed by treatment with PNGase F as indicated, and then analyzed by Western blotting using mAb 2D3 or anti-C9 tag antibody.
Computed tomograpgy (CT) (Inveon, Seimens) radiographic imaging was performed on some mice, as indicated, and resultant images analyzed in a blinded fashion using Inveon Research Workplace 4.2 visualization/analysis software and ImageJ.
