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Add indicated amounts of appropriate working standard (100 or 1000 µg/mL) to a 10 mL volumetric flask; bring to volume with the extraction solvent.
In addition, some unofficial reports in southeastern Virginia indicated amounts of up to 8 inches (200 mm) of rain.
In general, indicated amounts of PrP were diluted in saturating solution and incubated for 2 h.
The indicated amounts of cell lysates were separated by SDS-PAGE and were analyzed by immunoblotting as described [18].
The indicated amounts of protein were loaded onto 10% SDS-PAGE, transferred to nitrocellulose filters and blotted against different proteins using specific antibodies against the phosphorylated form or the total protein.
After overnight incubation, cells were induced with 500μM MgSO4 and indicated amounts of resveratrol for 4hrs.
The indicated amounts of vectors were mixed in OPTI-MEM medium (Gibco) with Lipofectamine 2000 (Invitrogen).
Transfections were performed with Fugene 6™ (Roche, Basel, Switzerland) using indicated amounts of the respective plasmids.
The indicated amounts of fosfomycin were added to each disk from a 50 mg/mL stock.
The indicated amounts of Ssa proteins were subjected to SDS-PAGE and stained with CBB.
About 1 μg of different ubiquitin species or indicated amounts of WT, S65A or ubiquitinPhospho-Ser65 was added as indicated.
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CEO of Professional Science Editing for Scientists @ prosciediting.com