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Cumulative release profiles indicated amounts released were approximately 68 80% of the initially loaded DNA.
Add indicated amounts of appropriate working standard (100 or 1000 µg/mL) to a 10 mL volumetric flask; bring to volume with the extraction solvent.
In addition, some unofficial reports in southeastern Virginia indicated amounts of up to 8 inches (200 mm) of rain.
In general, indicated amounts of PrP were diluted in saturating solution and incubated for 2 h.
The indicated amounts of cell lysates were separated by SDS-PAGE and were analyzed by immunoblotting as described [18].
For inhibition with the monovalent Anti-MUC1* Fab, indicated amounts (Fig. 4C) of the antibody were added immediately before adding the NM23 protein.
After overnight incubation, cells were induced with 500μM MgSO4 and indicated amounts of resveratrol for 4hrs.
The indicated amounts of vectors were mixed in OPTI-MEM medium (Gibco) with Lipofectamine 2000 (Invitrogen).
Transfections were performed with Fugene 6™ (Roche, Basel, Switzerland) using indicated amounts of the respective plasmids.
The indicated amounts of fosfomycin were added to each disk from a 50 mg/mL stock.
Lipids were diluted in chloroform and indicated amounts spotted onto PVDF membranes [ 25].
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