If using relative offsets, the whole plot is shifted by the indicated amount.
About 0.2 ng probe and indicated amount of proteins purified from Escherichia coli were mixed in 20 μl binding buffer (25 mM HEPES-KOH (pH 8.0), 1 mM DTT, 50 mM KCl and 10% glycerol).
Human macrophages were incubated for 24 h with media containing indicated amount of particles before infection with Mycobacterium smegmatis.
Murine or human macrophages were incubated for 24 h with media containing indicated amount of magnesium particles (Mg), magnesium corrosion particles (MCP) or cobalt chromium molybdenum particles (CCM).
Each sample (indicated amount of total proteins was resolved by SDS-PAGE.
To liberate untagged target proteins into the supernatant fractions, 300 500 µL lysis buffer was added to the Ni2+-NTA beads containing CPD-His6 fusion proteins and the indicated amount of inositol hexakisphosphate (InsP6, Calbiochem) was added.
The calcium phosphate method was used to transfect 0.5 µg of HIV-CAT, 50 ng of SV40-LUC as an internal control for transfection efficiency, and the indicated amount of either pcTat or a CMV-driven empty vector.
no addition; addition of the indicated amount of additives.
PCR fragments and genomic DNAs were used as templates for ligation in the indicated amount.
The indicated amount of DNA in Tetrahymena was determined for vegetative cells.
The indicated amount of protein lysate was separated by SDS-PAGE and transferred to Immobolin-P.
