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Medium supplemented with 10% serum or indicated agent was added to the outer cup.
A group of 6 rats were induced cancer and indicated agent (Table 1) was administered intra peritoneal from day-1 of post-induction for a period of 6-days and rats were sacrificed at day-6 and the relevant parameters were estimated in liver, kidney and urine.
One day after plating on coverslips, the cells were treated with empty vehicle or indicated agent for appropriate times.
Since these patients are likely to have to take medication throughout their lives, the efficacy of the indicated agent should be balanced against its safety and tolerability, which are also associated with non-adherence in patients with bipolar disorder [ 53].
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Pancreatic cell cultures were seeded into 24-well plates and treated with DMSO or indicated agents.
After 24 h incubation, the cells were washed twice with PBS and incubated with fresh serum-free DMEM containing all the supplements and indicated agents.
Following incubation, the cells were washed twice with phosphate-buffered saline (PBS) and then maintained in a serum-free DMEM including all supplements and indicated agents.
And then, the cells were washed twice with phosphate-buffered saline (PBS) and maintained in a serum-free medium including all supplements and indicated agents.
Cells were plated into the upper chamber in reduced serum DMEM (0.1%) with complete DMEM in the lower chamber and indicated agents added to both chambers.
Following incubation, the cells were washed twice with phosphate-buffered saline (PBS) and then maintained in a serum-free α-MEM including all supplements and indicated agents.
In Laurel, an F.B.I. document indicates, agents found a duffel bag containing pilot headsets and other aviation items.
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