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To isolate naive CD8+ T cells, spleens were harvested from uninfected mice of indicated age groups.
(C ) Q-body clearance time of individual cells of the indicated age groups, (old: average 9.2 generations, age between 6 and 19, N = 50; young: 0.4 generations, age between 0 and 1, N = 70).
(D ) Primary hepatocytes were isolated by collagenase perfusion from YapKIAlb-Cre mice of indicated age groups, and percentage of GFPhigh hepatocytes was determined by flow cytometry; n ≥ 11. (E ) Mice were injected with BrdU for 3 consecutive days and liver sections were stained with BrdU and GFP antibodies.
Because individuals of all ages would be expected to benefit from quadrivalent influenza vaccines, there is a strong rationale for providing quadrivalent influenza vaccines to all indicated age groups, as long as the incremental cost relative to trivalent vaccines is not excessive.
(I ) Percentages of cells of indicated age groups that contain the indicated fluorescent foci, (N = 18 71 per group).
(C ) Quantification of Hsp104-foci containing cells in indicated age groups, (N = 317 1020 cells per genotype).
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For age during follow-up, we estimated effect modification by adding an interaction term between exposure and a time dependent categorical variable indicating age group (<60, 60-74, >75).
However, recent research indicated that age groups (<65 and ≥65 years) was not associated with survival time, with median survival 13 months and 15 months, respectively [ 4].
The left y-axis indicates the proportion belted, the right y-axis indicates the proportion who died, and the horizontal x-axis indicates the age groups.
Let Z2= η1,η2) be the dummy variables indicating three age groups, where η1=1 if the age is in the range 1.5 4, η1=0, otherwise, and η2=1 if the age is in the range 5 9, η2=0, otherwise.
The colors indicate the age groups: blue for children, green for adults and yellow for elderly/retired (E/R) individuals (Figs. 1).
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