Indeed, EphA2-deficient vascular endothelial cells fail to migrate and assemble in response to angiogenic cues in vitro [ 46] and are unable to incorporate into tumor blood vessels when they are co-transplanted with tumor cells in vivo [ 45, 47], indicating a critical function for EphA2 in tumor angiogenesis.
On the other hand, most substitutions of this residue abolished the activity of the lysozyme indicating a critical role for the function of the enzyme [24].
This clearly indicates a critical role of YAO function in zygotic development and the patterning of the apical cell lineage, although the precise mechanism remains to be revealed.
As already indicated we chose to focus on the hypothalamus given that this region has higher than normal levels of most cytokines and the fact that most research indicates a critical role for hypothalamic functioning in cytokine induced neuronal alterations.
Yap CKO mice had robust increases in cardiomyocyte apoptosis and cardiac fibrosis that were associated with decreased cardiac function, indicating a critical role for Yap in maintaining adult heart homeostasis [122].
Comparison of protein sequences revealed that the Leu196 residue is invariant in mammals indicating a critical role for normal protein function.
Furthermore, a glycine and three cysteine residues were absolutely conserved in all DIA1-family proteins, indicating a critical role in protein structure and/or function.
We found that, in contrast to the partial inhibition of lamellipodia formation caused by Dock7 knockdown, depletion of Dock7 abolished HGF-induced cell spreading, measured 60 min after HGF treatment, indicating a critical role for Dock7 in this function.
Our genetic data indicate a critical role of ubiquitination for D-cbl function, in accord with biochemical models.
Our genetic data also indicate a critical role of ubiquitination for D-cbl function, consistent with biochemical models.
