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Red bars indicate reads from Miseq run of samples normalised using the Sequalprep (Invitrogen) plate-based normalisation kit.
Green indicates read count in cell line two is at least two-fold of that in cell line one.
Red indicates read count in cell line one is at least two-fold of that in cell line two.
It indicated reading for pleasure was more important to a child's development than how educated their parents were.
The 'other' category indicates reads not annotated to any of the Chicken non-coding RNAs in the Ensembl database.
For the genome alignment classes, NM indicates reads that could not be mapped to the genome, R* reads that were mapped to repeat areas and U0-U2, reads that were mapped with either no, one or two mismatches to the genome.
The strandedness of the RNA is also depicted: signal above the RIP-seq midline indicates reads mapping to the plus strand, signal below the RIP-seq midline represents the minus strand.
7+ETn and 7+60ETn indicate read-through transcription from an intact exon 7 into adjacent ETn sequences.
The entire probe was protected indicating read-through of the transcripts into the RP067 coding region.
Note that the blue directional spans above the coverage plot indicate single reads (not read-pairs) spliced across intron-sized gaps.
Both results indicate that read length correlates negatively with chimericity.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com