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Solid lines indicate reactions that are common in both yeast and mammals, whereas dotted lines are unique to yeast.
Arrows with shadows indicate reactions induced by light (hν), asterisks indicate excited species (electrons promoted to higher energy levels), single brackets indicate mineral surfaces, and dotted lines indicate surface adsorption.
Solid arrows stand for mass transfer reactions, i.e. reactions that consume reactants to produce products, whereas dashed arrows indicate reactions where the abundance of a species regulates a reaction rate, but the reactant concentration is not affected by the occurrence of the reaction.
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Solid lines indicate reaction pathways allowed in the model.
The color codes of the reactions indicate reaction rate values and it can be easily verified that all variable metabolites are at steady state for all flux modes.
Specifically, values of 1- P(X ≥ n12) that are smaller than a predetermined significance threshold would indicate reaction pairs with a tendency to not occur in the same metabolic network.
Where indicated, reactions contained 10 μM forskolin or 100 μM 4-hydroyestradiol (Steraloids, Inc).
Where indicated, reactions were supplemented with 10 pmol of in vitro transcribed CLTA1 sgRNA.
Where indicated, reactions were supplemented with [S]Met (135 mCi/mmol final) (Perkin Elmer, Waltham, MA) or varying amounts of [H]DHA (Perkin Elmer, Walthman, MA) as indicated.
A score above 20 indicates reactions of clinical importance and a score above 35 indicates severe symptoms with high a probability of a PTSD diagnosis [ 23].
Unless otherwise indicated, reactions were initiated by addition of purified recombinant NDH-2 or membrane preparations from cells overexpressing NDH-2.
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CEO of Professional Science Editing for Scientists @ prosciediting.com