Immunostaining studies using different subcellular markers demonstrated that mTORC1 is localized in enlarged vesicles that contain LAMP2, indicating its localization on the late endosomes.
Moreover, sequestosome-1/p62, a marker for autophagy impairment, is elevated in chronic smokers [6, 7] and its accumulation in the insoluble fraction indicates its localization to aggresome-bodies [6, 7].
Furthermore, our work identifies its homolog in higher plants from molecular point of view and indicates its localization in the thylakoid membrane.
Transient expression of MaPIP1 1-GFP fusion protein indicated its localization at plasMaPIP1 1-GFP
The overlay of AtGALT29A-mCer3 with the co-expressed Golgi marker protein, STtmd-YFP [ 25] indicated its localization to the Golgi apparatus.
The proteinase protection assay demonstrated the VLPs integrity and the absence of degradation of the fused antigen, thus indicating its internal localization.
Furthermore, in control cells, Cyt c was localized in the perinuclear-mitochondrial region, (Supplementary Figure S6A) whereas it acquired a diffused cytoplasmic localization, indicating its release from the mitochondria, in IAV NP-expressing cells (Supplementary Figure S6B).
Like these proteins, the functional role of H3 in the PV remains to be investigated, however, this localization indicates its "moonlighting function".
CpoB also failed to localize following inhibition of PBP3 with aztreonam, indicating that its localization also requires ongoing septal PG synthesis.
E protein was hardly detected without cell permeabilization, indicating that its localization was intracellular and was not expressed at cell surface (data not shown).
